2.3. Glucose tolerance test
At week 4, mice fasted for 8 h were given a 50% glucose solution in water (2 g/kg of body weight) by intraperitoneal injection, and a blood sample was taken from the tail vein before glucose administration and 15, 30, 60 and 120 min after. Blood glucose was determined with a handheld glucometer (Contour XT, Ascensia Diabetes Care, S.L., Barcelona, Spain).