Supplementary results
Supplementary Figure S1. Western blot representative bands of several markers involved in distinct pathways of apoptosis. (A) Extrinsic pathway, (B) intrinsic pathway and (C) Reticulum endoplasmic mediated pathway. (D) Scheme of the general mechanism of action of tigecycline in apoptosis induction. Data are presented as mean ± SEM.
Supplementary Figure S2. Effect of tigecycline and 5-FU on murine CAC model. (A) Survival rates. (B) Tumor volume. Representative Western blot bands of the different markers involved in (C) proliferation, (D) epithelial-mesenchymal transition and (E) apoptosis.
Supplementary Figure S3. Flow cytometry analysis of myeloid cells populations . (A) Gating strategy for the selection of different populations of immune cells and (B) percentage of each one. Data are presented as mean ± SEM. Bars with different letter differ statistically (P<0.05).
Supplementary Figure S4. Flow cytometry analysis of lymphoid cells populations. (A) Gating strategy for the selection of different populations of immune cells and (B) percentage of CD3+ and regulatory T cells. Data are presented as mean ± SEM. Bars with different letter differ statistically (P<0.05).