miR-29s suppress cytokine-induced expression of sST2 and IFNAR1
in BEAS-2B cells
A, Cytokine-induced soluble ST2 (sST2) release from BEAS-2B cells. A
human bronchial epithelial cell line, BEAS-2B cells, was stimulated with
the indicated concentrations of IL-4 and TNF-α, alone or in combination,
for 48 h. The protein concentration of sST2 in each culture supernatant
was measured by ELISA. B, Schematic representation of the gene
structures of miR-29 family members. C, The effects of inhibition or
overexpression of miR-29s on sST2 release from BEAS-2B cells were
examined. Following 24-h transfection of 10 nM of miRNA for miR-29
inhibitors, mimics or a negative control, the cells were stimulated with
a combination of 100 ng/ml IL-4 and TNF-α for 48 h. The protein
concentration of sST2 in each culture supernatant was measured by ELISA,
and the data are shown as the mean ± SD of triplicate samples. *P< .05 by Student’s t -test. D, Effects of inhibition or
overexpression of miR-29s on the expression of ST2 and IFNAR1 proteins
in BEAS-2B cells. BEAS-2B cells were treated as above in C. Whole-cell
lysates were subjected to Western blot analysis of the expression of
ST2, IFNAR1 and β-actin (as a loading control). The data are
representative of two separate experiments. Chr.: chromosome; NC:
negative control; IFNAR1: IFN (α, β and ω) receptor 1; ACTB: β-actin.