FIGURE LEGENDS
FIGURE 1 . Conjunctival microbiome compositions of healthy and
AC participants. A, Microbiome of healthy subjects (n = 48) and
AC patients with SAC/PAC (n = 21) or VKC (n = 18)
classified at the kingdom level. B, Microbiome of healthy subjects and
AC patients classified at the genus level.
FIGURE 2 . Taxonomic differences between individuals with AC
(n = 39) and healthy individuals (n = 48). A, Alpha
diversity measured with the Shannon index was computed for AC and
healthy samples. B, Beta diversity measured with Bray-Curtis
dissimilarity within AC and healthy samples. P values of alpha
and beta diversity were computed using a two-sided Wilcoxon test. C,
PCoA of samples from all 87 participants based on species-level
Bray-Curtis distance. D, LDA effect sizes of species differentially
abundant (LDA effect size > 4) in the participants with and
without AC. E, Relative abundance distributions of species enriched and
reduced in AC. Species with fold change > 5 were displayed.
Relative abundances in the heatmap were centered and scaled across all
the samples.
FIGURE 3 . Functional differences between individuals with AC
(n = 39) and healthy individuals (n = 48). A, KEGG
orthology, EC gene families, EggNOG clusters of orthologous groups, and
Pfam protein families that are enriched in AC patients versus healthy
subjects. Only the protein family signatures identified with LEfSe
(Kruskal-Wallis rank-sum test P < 0.05 and LDA effect
size > 2) are displayed in the scatter plots. The gene
families (enriched in AC) with LDA effect size > 3 are
highlighted in red. RP, ribosomal protein. B, Prevalence of CARD
antibiotic resistance genes in the conjunctival metagenome from patients
with AC and healthy individuals.
FIGURE 4 . Microbiome differences between individuals with
SAC/PAC and VKC. A, Alpha diversity measured with the Shannon index was
computed for healthy subjects (n = 48) and patients with SAC/PAC
(n = 21) and VKC (n = 18). B, Beta diversity measured with
Bray-Curtis dissimilarity within healthy, SAC/PAC, and VKC samples. C,
PCoA of samples from 39 AC participants with SAC/PAC or VKC based on
species-level Bray-Curtis distance. D, LDA effect sizes of species
differentially abundant (LDA effect size > 3) in SAC/PAC or
VKC.
FIGURE 5 . Relative abundance distributions of species
differentially abundant between individuals with SAC/PAC and VKC. A,
Overview of the distributions of species associated with the two types
of AC. Relative abundances in the heatmap were centered and scaled
across the samples from individuals with AC. B, Relative abundances of
the species enriched in SAC/PAC. C, Relative abundances of the species
enriched in VKC. Only the species that were present in >
50% samples with mean relative abundances > 0.1% in the
overabundant group were displayed in the B and C.