Figure legends
Figure 1. 2-MAGs percentage based on maximum theoretical yield
at different reaction times using TLL adsorbed on Sepabeads C-18 and TLL
commercial at studied reaction times and temperatures.
Figure 2. Reaction kinetics of 2-MAGs production from echium
oil at optimum temperature (25 °C) catalyzed by TLL commercial (a), TLL
adsorbed on Sepabeads C-18 (b) and TLL adsorbed on Sepabeads C-18 using
equivalent enzyme loading to commercial biocatalyst (c).
(●) TAGs, (▲) DAGs, (■) 2‑MAGs.
Figure 3. Fractionation of synthesized 2-MAG by SPE at optimal
reaction time analyzed by HPLC‑ELSD: standard mixture of different
species of lipids (a), original reaction (b), SPE fraction composed by
FAEEs (c) and SPE fraction composed by pure 2-MAGs (d).
Figure 4. Recovered activity (%) of TLL adsorbed on Sepabeads
C-18 and TLL commercial for 2‑MAGs production after five reaction
cycles. Reutilization was evaluated in reaction cycles of 2 h (optimal
reaction time).
Figure 5. Reaction kinetic of transesterification reaction
between synthesized 2-MAGs and caprylic acid ethyl esters catalyzed by
RML commercial (a) and RML adsorbed on Sepabeads C‑18 (b).
(●) STAGs, (▲) DAGs, (■) 2‑MAGs.
Figure 6. Chromatograms obtained by HPLC-ELSD of
transesterification reaction between synthesized 2-MAGs and caprylic
acid ethyl esters: reaction products at initial reaction time (a),
reaction products after 20 min (b) and reaction products after 1 h (c).