ATSW collection
ATSW was collected in two different seasons of the year; in winter (January 2019) and in autumn (October 2019) with an aseptic procedure. Using surgical gloves, 3000 ml of water was collected with a sterile 50 ml syringe, close to the thermal spring. The samples were poured into three 1 L containers for microbiological analysis (VWR) and transported as soon as possible for microbiological analysis and processed rapidly following collection. The in situ temperature of water, in winter’s sampling, was 55.4 °C and in situ pH was 6.8 - 7 whereas, in autumn’s sampling, the temperature was 56 °C and pH was still 6.8 - 7. Chemical composition of ATSW was reported inTable 1 .