Figure legends
FIGURE 1. Generation and characterization of VSV/RABV-GP. (A) VSV/RABV-GP production was measured by sandwich ELISA. Results have been expressed as the mean of OD450 ± SD values and are representative of at least three independent experiments. Dotted line indicates the background. (B) BHK21 cells were transduced with VSV/RABV-GP or VSV/VSV-GP in the presence or absence of the indicated antibodies. Virus entry was quantified by measuring the luciferase levels at 18 h post-transduction. Error bars represent the SD from the mean (n = 3). Statistical significance was assessed by Student’ s t -test. *, P<0.05, ***, P<0.001; ns, not significant.
FIGURE 2. Immunization schedule and humoral immune response induced by vaccination. (A) Schematic diagram of the immunization protocol. BALB/c mice were i.p. immunized with PBS, RABA, or VSV/RABV-GP at 0 and 14 days after the first immunization. At 28 days post immunization, mice were sacrificed and serum samples were collected. (B and C) Detection of the levels of RABV GP specific IgM (B) and IgG (C). Results are expressed as the mean (n=3) of OD450 ± SD values and are representative of at least three independent experiments. Statistical significance was assessed by Student’ s t -test. ***, P<0.001; ns, not significant.
FIGURE 3. Influence of VSV/RABV-GP immunization on the type of T helper cell response. The serum specific IgG1 (A) and IgG2a (B) titers were determined as indicators of a Th2 or Th2 type of response, respectively. (C) IgG1/IgG2a titer ratios of immunized mice. Results are expressed as the mean (n=3) of OD450 ± SD values and are representative of at least three independent experiments. Statistical significance was assessed by Student’ s t -test. ns, not significant.
FIGURE 4. Protective efficacy of VSV/RAVB-GP vaccination.Detection of neutralizing antibody titers in the serum of the immunized mice by fluorescent antibody virus neutralization test (FAVNT). Results are expressed as the mean (n=3) of serum neutralizing titers ± SD values and are representative of at least three independent experiments. Statistical significance was assessed by Student’ s t -test. *, P<0.05.