3.3 VSV/RABV-GP induces a T helper cell type-2 (Th2) immune
bias
To determine whether Th1 and/or Th2 humoral response was induced by
vaccination with VSV/RABV-GP, the levels of specific IgG1 and IgG2a
subclasses were compared with the inactivated RABV vaccine. Sera samples
showed statistically indistinguishable GP-specific IgG1 and IgG2a
responses (Figure 3A and B). Analysis of the antigen-specific IgG1/IgG2a
ratio in the sera samples revealed that mice immunized with VSV/RABV-GP
had a greater IgG1/IgG2a than the inactivated RABV (Figure 3C). The data
indicate that VSV/RABA-GP induces a Th2-biased immune response.