2.3 Mouse immunization and sample collection
Eight-week-old BALB/c mice were randomized into three groups. All groups
were vaccinated intraperitoneally twice at 2 week intervals. Mice in
group 1 were given PBS and 50 μL Alum adjuvant as controls. Mice in
group 2 were given 10 μg of VSV/RABV-GP and 50 μL Alum adjuvant. Mice in
group 3 were given 10 μg of inactivated RABV and 50 μL Alum adjuvant. At
28 post-initial immunizations, serum samples were collected.