2.5.3 Cell lysis
Samples from microbioreactor experiments were taken at the end of each passage. Samples from fed-batch cultivations were taken every 2 hours and samples from chemostat cultivations every 24 hours. The sample volume for intracellular protein quantification was calculated as 3.5/OD600 = volume in mL, which corresponds to ~1 mg CDM. Cell lysis for quantification of intracellular recombinant proteins was performed as described elsewhere.[28]