2.5.1 Flow cytometry
A CytoFLEX S flow cytometer (Beckman Coulter, Brea, USA) was used to monitor subpopulations of GFPmut3.1-producing cells. Cells were diluted 1/2025 in PBS. Excitation of GFPmut3.1 fluorescence was performed at 488 nm, with subsequent emission measured using the FL1 Channel (525BP40-A). Data were recorded for 15,000 cells per sample at ~300 events/s and were analyzed by Kaluza analysis software (Beckman Coulter).