3.3 Long-term stability of select derivatives in chemostat
cultivations
The derivatives described above were generated under carbon-limited
fed-batch-like production conditions in the microbioreactor. This raised
the question about how select derivatives that can grow unhindered
despite recombinant protein production behave in long-term cultivations
and whether they can continue to maintain productivity over several
generations.
To address this question, we performed comparative lab-scale chemostat
cultivations. In the case of the easy-to-produce protein GFP, the best
mutant B3<T7-GFP> #E7P6 S10, which had a
mutation in the pLac promoter, was compared to the non-mutated wild-type
strains BQ<A1-GFP> wt and
B3<T7-GFP> wt in long-term chemostat
cultivations.