2.5.3 Cell lysis
Samples from microbioreactor experiments were taken at the end of each
passage. Samples from fed-batch cultivations were taken every 2 hours
and samples from chemostat cultivations every 24 hours. The sample
volume for intracellular protein quantification was calculated as
3.5/OD600 = volume in mL, which corresponds to
~1 mg CDM. Cell lysis for quantification of
intracellular recombinant proteins was performed as described
elsewhere.[28]