3.3. Field sample evaluation of the VNUA-P54 real-time PCR assay
A total of 187 field clinical samples including whole blood (n=122), serum (n=12), spleen (n=37) and kidney (n=16) collected from pigs displaying ASF- clinical signs were tested by both VNUA-P54 and Tignon real-time PCR assays. The results showed that both assays were able to detect ASFV in all sample types. The mean Ct value of VNUA-P54 assay was 19.70 and Tignon assay was 20.83. Diagnosis results according to the type of samples showed that the mean Ct values obtained from spleen, kidney, serum, and whole blood samples were 19.01, 19.78, 24.42, and 19.44 for VNUA-P54 assay and 20.25, 20.91, 24.65, and 20.63 for Tignon assay, respectively (Table 2).