3.2. Analytical specificity of the VNUA-P54 real-time PCR assay
To determine the analytical specificity of the VNUA-P54 real-time PCR assay, a total of 15 different ASFV reference strains of p72 genotypes I, II and V were used and the result showed that the VNUA-P54 real-time PCR assay was able to detect all of them. The specificity of the VNUA-P54 real-time PCR was also tested with other swine virus strains of CSF, FMD, PRRS, and PED and the assay did not detect any of those pathogens (Data not shown).