Orthotopic lung cancer model in mice
Mice were anesthetized with chloral hydrate (300 mg/kg) via intraperitoneal injection and placed in the right lateral decubitus position. A total of 1 × 106 cells in 50 µL RPMI-1640 medium and 50 µL Matrigel were injected into the left lung parenchyma through the left rib cage. Mice were monitored daily. Mice were euthanized by pentobarbital sodium, and the lung tissues were removed.