During the experiments, samples were taken from both phases and analyzed
in a Perkin Elmer Clarus 500® (PerkinElmer Inc.,
U.S.A.) gas chromatograph equipped with a 30 m SLB
SUPELCO® (Supelco, Inc., U.S.A.) column with a 0.25 mm
x 0.25 µm cross section, thus generating concentration profiles of all
components over time. Exemplary for our experimental results, figure 4
shows the concentration profiles of the intermediate COH and the final
product CCI in the organic phase of experiment 2 as well as the
corresponding simulated values using the mathematical model described in
the previous chapter. The experiment shown in figure 4 has a total
duration of 80 hours: during the first 65 h, the aqueous cycle is
running without the organic cycle, allowing for a separate evaluation of
the reactions as well as sufficient intermediate formation leading to a
clearer concentration profile in the later connected organic cycle.