TMUV genetic diversity and epidemiological data in China
To investigate the genetic diversity of TMUV, we used three different
methods (NJ, ML, and MCC) to construct the phylogenies using the ORF
sequence of the TMUVs. All of the strains represent four different avian
species that were isolated in China from 2010 to 2019, and the strain
XZW10694 (Accession number: KY937189) as outgroup. Three different
clades were observed using the three different methods (Fig.2a, 2b, and
2c). Compared with a previous study (Dai, Li, & Tao, 2015), a new clade
was observed in the three different phylogenetic trees. This new clade
(clade 3) was isolated in Shandong in 2014 and the host was Anas
platyrhynchos (Accession number: MH748542). Clade 3 showed a longer
evolutionary distance compared to clades 1 and 2, which might suggest
that clade 3 is a potential new genotype. Based on the BSP, the TMUV
might have diversified from 2010 to 2012, after which it entered the
platform period (Fig. 3).