Origin of TMUV in China
To trace the origin of TMUV, we constructed an ML tree based on the ORF
gene sequence (Fig. 1a). We found that all the avian TMUV strains were
closely related to the mosquito TMUV with high confidence. The West Nile
virus lineage 2 (NC001563) was selected as outgroup of the mosquito TMUV
and avian TMUV strains, which might indicate a potential mosquito TMUV
origin. To further verify this result, an MCC tree was constructed (Fig.
1b). The MCC tree showed a topology similar to that of the ML tree.
Although this provides evidence that a close relationship exists between
avian TMUV and mosquito TMUV, it is worth noting that only 3 strains of
mosquito TMUV could be obtained from GenBank; we will update the
analysis once more mosquito TMUV is available.