3.4.3 Comparison with HPLC results
In order to compare the results obtained from the qHNMR technique, the prepared standard samples with different MO and EMO molar ratios were analyzed by an HPLC technique. In the HPLC chromatogram, the retention time of MO and EMO were found to be 7.92 and 8.38 minutes, respectively. In the HPLC chromatogram of standard mixtures of MO/EMO, percentage peak area values were calculated (Table 1 ) and was found to be directly proportional to the percentage molar concentration of the compounds (Fig 2s –5s , supporting information). The results of EMO molar concentration obtained from HPLC when correlated with that obtained from the qHNMR technique showed acceptable agreements affirming the validation of the qHNMR method (Fig. 5 ).