To the Editor,
This letter is a response to the commentary by Dr. Finsterer (Finsterer,
2020) on our paper entitled “Homozygous mutations in C1QBP as
cause of progressive external ophthalmoplegia (PEO) and mitochondrial
myopathy with multiple mtDNA deletions” (Marchet et al. 2020). Here we
try to address the key concerns raised by him.
We did not make any distinction between pure PEO and PEO plus, but just
considered PEO as a clinical sign which is extremely useful to suggest
the presence of a mitochondrial disorder, in particular of primary
mitochondrial myopathy (PMM). We stressed the use of the term PEO
because it is the one used in OMIM to describe these genetic diseases,
with 10 entry genes classified as “Progressive external ophthalmoplegia
with mtDNA deletions” (Phenotypic Series - PS157640). Moreover, we
stated that PEO usually (and not necessarily) starts with ptosis;
indeed, the two patients with C1QBP mutations we described (P1
and P2) presented with ptosis at disease onset. Accordingly, the
consortium on Trial Readiness in Mitochondrial Myopathies confirmed that
“the most common presentation of PMM is chronic PEO” and that “PEO is
usually accompanied by bilateral eyelid ptosis, which is often the
presenting symptom” (Mancuso et al. 2017).
In the first paper about C1QBP mutations (Feichtinger et al.
2017), all the four reported patients presented with cardiac involvement
leading the authors to sustain in the title that biallelic C1QBPmutations cause severe neonatal-, childhood-, or later-onset
cardiomyopathy. Although we cannot exclude subtle cardiac dysfunction in
our patients (since they did not undergo long-term ElectroCardioGram
recordings, trans-esophageal echocardiography, or cardiac MRI), their
standard ECGs and echoCGs were normal and thus we still consider valid
the main message of our paper: subjects with C1QBP mutations may
present with adult-onset PEO/PMM phenotype, without overt
cardiomyopathy.
We obviously agree that mtDNA genetics is peculiar, and that different
level of heteroplasmy may explain the variable phenotypic expression of
mtDNA mutations, but we are talking here about mutations in a nuclear
gene with an autosomal recessive inheritance. No DNA from any family
members was available for segregation studies but we expect that the
parents of P1 and P2 (reported to be second-grade and third-grade
cousins, respectively) would have tested as heterozygous carrier. Both
patients have no siblings. Anyway, detailed clinical investigations of
first-degree relatives, not harboring the homozygous C1QBPmutation, would be not informative and hence, in our opinion, useless.
The presence of multiple mtDNA deletions is a secondary effect of the
mutations in C1QBP , although the exact mechanism linking C1QBP
with mtDNA maintenance and stability is not known. The assertion that
residual protein amount and different localization of the C1QBPmutations could explain the variable observed phenotypes, including both
clinical symptoms and molecular/biochemical defects (mtDNA deletions,
mitochondrial respiratory chain - MRC - complex activities,
histochemical staining) remains plausible. As suggested by Dr.
Finsterer, it is possible that the phenotypic variability ofC1QBP variants is fairly attributable to variable heteroplasmy of
secondary mtDNA deletions and/or mtDNA copy number, but it is not
possible to test this hypothesis in detail (e.g. throughout assessment
of heteroplasmy in different muscle types, including extraocular muscle,
and at different time points during disease progression). All the
experiments reported in our paper (Marchet et al. 2020) were performed
on a single muscle biopsy from quadriceps of the two patients.
Densitometry analysis of the Southern blot (Fig. 1C reported in Marchet
et al. 2020) revealed 58% and 48% mtDNA deleted species in P1 and P2,
respectively. Nevertheless, an exponential accumulation of multiple
mtDNA deletions has been reported in post-mitotic tissues during aging
(Cortopassi et al. 1992), and thus we cannot exclude an influence of the
age at biopsy and of duration from onset disease on this result.
Another important issue is related to the presence of mosaic of cells in
the same tissue, which is expected also in our patients based on
histological analyses showing fibers with different features likely
related to different levels of mtDNA deletions. Accordingly, previous
single-cell analysis has revealed that mtDNA deletions are distributed
as a mosaic of affected and non-affected cells (He et al. 2002). While
the link between heteroplasmy level and biochemical/clinical phenotype
is well established in patients with single large‐scale mtDNA deletion,
it is more complex in patients with multiple mtDNA deletions, where each
muscle fiber may contain different, and more than one, mtDNA deleted
species (Lehman et al. 2019).
Regarding mtDNA copy number, we did not assess it directly but we expect
the same limitations reported above, because of experimental data from a
single specimen characterized by intercellular heterogeneity.
Nevertheless, some indirect indications can be obtained by already
reported histological and biochemical findings. Muscle cells with high
levels of mtDNA deletions typically show mitochondrial proliferation as
compensatory mechanism, which is reflected by the presence of Ragged Red
Fibers (RRF). Moreover, the activity of citrate synthase (CS) is often
used as a quantitative marker for mitochondrial mass. In both P1 and P2,
we observed the presence of many RRF but the CS activity in total muscle
homogenate was in the normal range (118% and 100% of the controls’
mean for P1 and P2, respectively) again confirming variable heteroplasmy
in different fibers but indicating an overall normal amount of
mitochondria and, roughly, of mtDNA copy number.
All the above considerations are useful to explain also the last concern
by Dr. Finsterer, i.e. why biochemical investigations of P2 were normal.
Notably, the histochemical staining for cytochrome c oxidase (i.e.
complex IV) was decreased in scattered fibers from P2, despite
biochemical assay showed normal values for MRC complexes. It has already
been reported that the activities of respiratory complexes in muscle
from PEO patients range from normal to about 50% of the controls’ mean
(Viscomi & Zeviani, 2017). Likewise, normal MRC activity has been
observed in several patients presenting with mtDNA deletions caused by
mutations in nuclear genes (e.g. POLG, POLG2, RNASEH1 …).
More recently, by single cell studies some authors demonstrated that
genetic defects do not strictly correlate with MRC deficiency in fibers
with multiple mtDNA deletions (Lehman et al. 2019).
In conclusion, the very limited number of C1QBP cases reported up
to now and their allelic heterogeneity hamper to define any
genotype–phenotype correlations, but nevertheless indicate a huge
clinical spectrum associated with C1QBP mutations, ranging from
early-onset severe cardiomyopathy to adult-onset PEO/PMM.
CONFLICT OF INTERESTS
The authors declare that there are no conflicts of interests.