Figure 2. (A) Shannon index estimates of species diversity across a successively rarefied shotgun metagenomic sequencing dataset, in which reads could be classified as bacterial derived, and (B) Pearson correlation coefficients from Mantel tests of species Bray-Curtis dissimilarity matrices created from a minimally rarefied (9.3 million bacterial read-pairs) versus successively rarefied Nextera XT prepared shotgun metagenomic sequence datasets from Sable Island horse fecal samples.