Data and Statistical Analysis
All data and statistical analysis comply with the recommendations on experimental design and analysis in pharmacology (Curtis et al., 2018). Data are presented as mean + standard errors of the mean (SEM). Statistical differences between treatments were determined by analysis of variance (ANOVA) followed by Tukeys multiple comparison post-hoc tests where appropriate. One-way ANOVA were used for three of more unmatched groups. Two-way ANOVA were used to analyse data when response was influenced by two independent factors of interest. All statistical analyses were performed using GraphPad PrismTM for Microsoft Windows® (Versions 8, Graphpad software®, USA) where p <0.05 was accepted as significant for all cases.