Gas exchange analysis in plants with reduced guard cell
NtSUS2 expression
Only L3 displayed lower A compared to WT in either light or
CO2 response curves determined in well-watered plants
(Figures 2A,B). The data from these curves also revealed that no
differences in the steady state values of transpiration rate (E ),
dark respiration (Rd) and intrinsic WUE (iWUE)
(A /g s ratio) were apparent between WT and
transgenic lines, whilst L3 and L13 displayed reduced steady state
stomatal conductance (g s)
compared to WT plants (Figures 2C-F). We also evaluated the kinetics of
gas exchange during the dark-to-light transition (Figures 3, S2). No
statistical difference was observed in A between WT and
transgenic lines (Figure 3A). The light stomatal response was, in
general, slower in the transgenic lines, which alter the dynamic of bothiWUE andC i/C aand leads to significant reductions in Sl max in
L3 and t50% in both L3 and L13 (Figures 3B-F).
Interestingly, the dynamic of WT g s curves have a
decrease in g s after 2000 sec, and this was not
observed in the transgenic lines (Figure 3B).