Figure 2. TEC protects mice from 0.1% DDC diet-induced liver injury and inflammation. C57BL/6J mice were fed with 0.1% DDC and/or TEC for 1 week, and then sacrificed at the end of the experiment. (A) Serum level of AST, ALT, γ-GT, and AP were measured by ELISA kits, n=6. (B) Representative images of hepatic HE staining are shown. (C) Representative images of hepatic Sirius red staining are shown. (D-E) D: liver-isolated immune cells were stained for CD11b and F4/80 and analyzed by flow cytometry, representative dot plots are shown. E: further quantification of flow cytometry. (F-G) Hepatic mRNA levels of TNF-α, IL-1β, Col1α1, TIMP-1 and MMP9 were measured by qRT-PCR, n=5. *p<0.05. The data represent the mean ± SD.