Flow cytometry
KCs were incubated with anti-CD16/CD32 (BD 553141) FC receptor for 5 min at RT. And then cells were labeled with the following antibody at indicate dilutions. CD45-APC cy7(BD 557659),CD11b-FITC(BD 557391),F4/80-PE(BD 565410),after the 30 minute incubationat 4℃. KCs were washed and fixed in stain buffer FBS (BD 554656). Finally, analyses were performed on a Beckman DXFLEX Flow Cytometer.