Cell Culture
Primary KCs were prepared from the livers of 8- to 9-week-old male C57BL/6 mice and cultured in 10% FBS RPMI 1640 medium .A single-cell suspension form livers was prepared as described previously[53]. BMDMs were isolated from femoral and tibia bone marrow and then cultured in RPMI1640 medium(10%FBS) with 100 ng/mL M-CSF (Peprotech) for 5 days to fully differentiate macrophages. Primary mouse hepatocytes were obtained from the livers of male C57BL/6J mice (8 weeks of age) and cultured as previously reported[54]. HepG2 cells, a human hepatic cell line, were purchased from ATCC (Manassas, VA, USA, RRID: CVCL 0027) and cultured in DMEM medium containing 10% fetal bovine serum (FBS), 50 mg/mL penicillin/streptomycin and 2 mmol/L glutamine. Cells (~90% confluence) received treatment in serum-free medium.