Figure 3. TEC restored ANIT and DDC-induced hepatic bile acid metabolic dysfunction. (A-C) Liver, serum and feces samples were collected from ANIT and/or TEC treatment mice in Figure 1. A: bile acid pool size in the liver, serum and feces, n=6, was determined by ELISA kits. B: bile acid uptake (Oatp, Ntcp), bile acid export (Mrp2, Bsep) and sterol transporters (ABCG5 and ABCG8) genes were determined by qRT-PCR, n=5. C: western blot analysis was performed to determine the protein level of FXR and LXR in mouse liver, and quantitative statistics are shown in the right panel, n=5. (D-F) Liver, serum and feces samples were collected from DDC and/or TEC treated mice in Figure 2. D: bile acid pool size in the liver, serum and feces, n=5, was determined by ELISA kits. E: bile acid uptake (Oatp, Ntcp), bile acid export (Mrp2, Bsep) and sterol transporters (ABCG5 and ABCG8) genes were determined by qRT-PCR, n=5. F: western blot analysis was performed to determine the protein level of FXR and LXR in mouse liver, and quantitative statistics are shown in the right panel, n=5. *p<0.05. The data represent the mean ± SD.