Conjugated diene value (CDV)
The AOCS official method (Ti
1a-64) was used to determine CDV (AOCS, 1990). In brief, approximately
10 mg oil samples were weighed in test tubes (Pyrex, 16 × 125 mm) and
dissolved in 10 ml isooctane. The
solution (1.0 ml) was transferred to another test tube and diluted with
9 ml isooctane. The solution was added in a quartz cuvette and the
absorbance was measured with the same spectrophotometer used for PV at
233 nm. Three experimental replicates were analyzed in duplicate.