SNV annotations
Associated variants from the GWAS and GRS analysis were annotated using the Ensembl Variant Effect Predictor to determine their potential impact on gene transcription and translation (e.g., transcript expression, splicing, protein sequence) as well as reported associations with traits and diseases.(33) Non-coding variants were assessed for potential regulatory effects (i.e. expression quantitative trait loci) using RegulomeDB and Genotype-Tissue Expression (GTEx) databases.(34,35)