Eq. 2
Here, \(p_{H_{2}O}\) is the partial pressure of water vapour in the incubator, RH is the relative humidity (60%), and is the total pressure of water vapour at 35.5 °C. Once the water vapour conductance was calculated for these conditions, the water loss was calculated for the standard conditions (25°C and 0% humidity) using the formula developed by Ar et al., (1974):
\({\dot{M}}_{H_{2}O}=c*D_{H_{2}O}*\frac{\text{Ap}}{L}*P_{H_{2}O}\ \)