2.5. Artificial templates used as positive amplification control
(PAC)
The artificial templates used as PAC were cloned recombinant plasmids
carrying PCR amplicon of the target viruses. The PACs used for the CaPV
(Das et al. 2012) and PaPV (Das et al. 2017a) qPCR assays were reported
earlier. The PAC used for the FMDV RT-qPCR assay was an in-vitrotranscribed RNA derived from a cloned plasmid as described previously
(Wang et al. 2020). The purified PACs (plasmid DNA or in-vitrotranscribed RNA) were quantified on NanoDrop 2000c spectrophotometer
(Thermo Fisher Scientific) and used as template in the LOD (limit of
detection) studies.