2.8 Analytical specificity and diagnostic sensitivity: multiplex
assays
The analytical specificity was assessed against both target and
differential viruses. The CaP rule-out assays were tested against the
target (CaPV) viruses using CC isolates of
LSDV, GPV and SPV and CS of PaPV
(BPSV and ORFV), and differential viruses using CC isolates of BHV and
PPRV. The FMD rule-out assays were tested against the target (FMDV)
viruses using CC isolates of the seven serotypes of FMDV (A, Asia1, C,
O, SAT1, SAT2 and SAT3), CS of PaPV (BPSV and ORFV), and differential
viruses using CC isolates of BVDV, VSV and BTV.
The DSe of the multiplex assays was assessed against thirty-five CaPV,
thirty-six PaPV and thirty-nine FMDV specimens from experimentally
infected (CaPV and FMDV) or naturally infected (PaPV) animals as
described in the materials and methods.