2.8 Analytical specificity and diagnostic sensitivity: multiplex assays
The analytical specificity was assessed against both target and differential viruses. The CaP rule-out assays were tested against the target (CaPV) viruses using CC isolates of LSDV, GPV and SPV and CS of PaPV (BPSV and ORFV), and differential viruses using CC isolates of BHV and PPRV. The FMD rule-out assays were tested against the target (FMDV) viruses using CC isolates of the seven serotypes of FMDV (A, Asia1, C, O, SAT1, SAT2 and SAT3), CS of PaPV (BPSV and ORFV), and differential viruses using CC isolates of BVDV, VSV and BTV.
The DSe of the multiplex assays was assessed against thirty-five CaPV, thirty-six PaPV and thirty-nine FMDV specimens from experimentally infected (CaPV and FMDV) or naturally infected (PaPV) animals as described in the materials and methods.