mAb aggregates concentration
mAb aggregate concentration (% in mAb) was determined by analytical
size exclusion chromatography (SEC-HPLC) performed on a chromatography
system Prominence LC-20 (Shimadzu Corporation) using a TSKgel G3000SWxl
5 μm column (Tosoh Bioscience, LLC) with a mobile phase of 100 mM
potassium phosphate with 200 mM NaCl, pH 7.0.