Figure 1: Experimental design showing A) Setup, B) Custom made 3D printed spacer, C) Experimental timeline.
Figure 2: Representative echocardiographic images obtain with our 3D printed set-up in working mode during ESHP. ESHP, ex situ heart perfusion; LV, left ventricular.
Figure 3: Left ventricular functional assessment showing A) EF, FAC and FS, B) Strain. Error bars show the standard deviation. SAM, pump supported working mode; PAM, passive afterload working mode. LV, left ventricular; EF, ejection fraction; FAC, fractional area change; GLS, global longitudinal strain; GCS, global circumferential strain; RAD, radial strain; FS, fractional shortening.
Figure 4: Metabolic parameters measured. A) Aortic pH, B) Aortic lactate, C) Venoarterial lactate. Error bars show the standard deviation. SAM, pump supported working mode; PAM, passive afterload working mode.