Figure 1: Experimental design showing A) Setup, B) Custom made
3D printed spacer, C) Experimental timeline.
Figure 2: Representative echocardiographic images obtain with
our 3D printed set-up in working mode during ESHP. ESHP, ex situ heart
perfusion; LV, left ventricular.
Figure 3: Left ventricular functional assessment showing A) EF,
FAC and FS, B) Strain. Error bars show the standard deviation. SAM,
pump supported working mode; PAM, passive afterload working mode. LV,
left ventricular; EF, ejection fraction; FAC, fractional area change;
GLS, global longitudinal strain; GCS, global circumferential strain;
RAD, radial strain; FS, fractional shortening.
Figure 4: Metabolic parameters measured. A) Aortic pH, B) Aortic
lactate, C) Venoarterial lactate. Error bars show the standard
deviation. SAM, pump supported working mode; PAM, passive afterload
working mode.