Figure 5- Effects of temperature on circadian rhythms in phenotypic tails
We selected 10 accessions to represent the phenotypic tails of each trait as highlighted in Figure 1. Period, phase and RAE in these accessions were then re-quantified at 10°C, 16°C and 22°C to see if the tails remained diverged at lower temperatures. In Figure A, light grey lines show the mean periods of accessions in the long period tail group with the thick blue line showing the mean period for the whole group. Black lines show the mean periods for accessions in the short period group with the thick red line reflecting the mean for the whole short period group. In Figure B, light grey lines show the mean periods of accessions with high RAE (low robustness) with the thick maroon line indicating the overall high RAE tail mean. The black lines show the mean periods for accessions with low RAE (high robustness). The green line shows the overall low RAE tail mean. In both Figure A and B the dotted line is the mean for Col-0 at each temperature.
Figure C shows individual phase estimates from accessions belonging to the dusk-phased (dark filled point) or dawn-phased (open point) tail groups plotted as clock plots relative to 24/0 representing dawn. Tail group phase means are indicated by coloured arrows (dusk tail in orange and dawn tail in purple). Sample size contributing to accession mean at each temperature ranged from 14 to 24.)