Assay with recombinant P450 isoforms
Seventeen cDNA-expressed human P450 isoforms, including CYP1A1, CYP1A2,
CYP1B1, CYP2A6, CYP2B6, CYP2C19, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP2J2,
CYP3A4, CYP3A5, CYP3A7, CYP4F2, CYP4F3 and CYP46A1 were used.
Rivaroxaban (120 μM) was incubated with recombinant CYPs at 37 °C for 60
minutes to estimate the participation of the CYP isoforms. Results were
from at least three experiments carried out in duplicate. The
quantitative determination of metabolite concentration was as described
above.