Quantitative determination of phytohormones
Five of the six biological replicates were randomly selected in each
treatment, with a total of 20 samples measured. 0.3g (fresh weight) of
frozenādried leaf samples were grounded and extracted with 1 mL
methanol/water solution (80:20, V: V) two times. The extracted solution
was subsequently taken for quantification of JA, ET, and Pip. While the
concentrations of JA and ET were quantified with enzyme-linked immune
assays (ELISAs) (JA/ET ELISA kit, Fankewei, China), Pip concentration
was determined by the LC-MS method (Chen et al., 2018).