RT-qPCR
Reverse transcription quantitative polymerase chain reaction (RT-qPCR)
was performed by first extracting RNA via phenol-chloroform extraction
using TriReagent (Sigma-Aldrich, NSW, Australia) as per the
manufacturer’s instructions (Jianling Xie et al., 2020). Contaminating
DNA removal and reverse transcription were then performed on equal
amounts of RNA using QuantiNovaâ„¢ Reverse Transcription Kit. cDNA diluted
1:10 in nuclease free water was used as a template for qPCR reactions
with SYBR Green PCR Master Mix (Sigma-Aldrich). For a list of primers
used for qPCR reactions, see Supplementary Table S2.