RT-qPCR
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed by first extracting RNA via phenol-chloroform extraction using TriReagent (Sigma-Aldrich, NSW, Australia) as per the manufacturer’s instructions (Jianling Xie et al., 2020). Contaminating DNA removal and reverse transcription were then performed on equal amounts of RNA using QuantiNova™ Reverse Transcription Kit. cDNA diluted 1:10 in nuclease free water was used as a template for qPCR reactions with SYBR Green PCR Master Mix (Sigma-Aldrich). For a list of primers used for qPCR reactions, see Supplementary Table S2.