FIGURE 5 TA inhibits the STAT3 signaling pathway in
vivo . (a) Left: STAT3-regulated protein expression in tumor tissues.
The protein levels of STAT3, p-STAT3 (Tyr705), Survivin, Bcl-2, c-Myc,
ICAM-1, and Cyclin D1 in the tumor tissues were assessed using western
blotting. Right: Analysis of the results in (a). Protein levels were
quantified by using ImageJ software. Data are shown as means ± SD (n =
5). *P < 0.05. (b) Left: Immunohistochemical analysis
of tumor tissues. Tumor tissues were fixed, dehydrated, and embedded
into paraffin blocks. Tissue sections were cut from paraffin blocks
and immunolabeled to detect the expression of p-STAT3 (Tyr705), c-Myc,
Survivin, MMP-9, and ICAM-1. Scale bar, 50 μm. Right: Analysis of the
results in (b). Positive cells were counted by using ImageJ software.
Data are shown as means ± SD (n = 5). *P < 0.05.