Background: Improving patient safety is an increasing priority for health workers who have roles in perioperative care about patient safety. Also, patient safety initiatives aimed at creating a safe operating room culture are increasingly being adopted. This study aims to evaluate the attitudes of health workers who have roles in perioperative care about patient safety. Methods: The cross-sectional study was conducted in a state hospital in the eastern part of Turkey between January 2018 and March 2018 with the participation of 129 volunteer health workers including surgeons, surgical technicians, nurses and other health workers who have roles in perioperative care by using the SAQ-OR instrument. Results: An analysis of the relationship between SAQ-OR total and sub-scales score and age indicated a positive, significant relationship between Teamwork Climate, Job Satisfaction, Safety Climate, Working Conditions and SAQ-OR total scores (p<0.05). Besides, Job Satisfaction, Perceptions of Management, Safety Climate sub-scales and weekly working hours indicated a positive, statistically significant relationship (p<0.01). Conclusion: Participating health personnel’s attitudes towards operating rooms were found to be low. More attention should be attached on the issue by the hospital administration in order to improve patient safety culture which was not found at good levels.