2 | MATERIALS AND METHODS
In October 2018, FMD-like clinical presentations, including fever, hoof decay, and a limp, occurred in three buffaloes in a buffalo farm (n = 80) in Guangdong, China. Three oral swab fluids were collected by the farm owner and transported to the Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences using an insulated container with an ice pack. We extracted the nucleic acids from the oral swab fluids and used reverse transcription PCR to detect FMDV, VSV, BVDV, BTV, and BoHV-1 (Marlise et al., 2005) (Appendix Table) according to the reference protocols and SVV, including detection and genome amplification (Table 1). We used eight cell types from four different origins to isolate and purify the virus. We conducted a genetic evolutionary tree based on the SVV polyprotein with 1000 bootstrap replicates using MEGA6.06 software (Neighbor-Joining). Our results indicated that there was full-length nucleotide and amino acid sequence similarity in the alignment between SVA/GD/China/2018 and other 35 SVV strains published from China and other countries by MegAlign (DNAStar Lasergene.v7.1) software using Clustal W.