2 | MATERIALS AND METHODS
In October 2018, FMD-like clinical presentations, including fever, hoof
decay, and a limp, occurred in three buffaloes in a buffalo farm (n =
80) in Guangdong, China. Three oral swab fluids were collected by the
farm owner and transported to the Animal Disease Diagnostic Center,
Institute of Animal Health, Guangdong Academy of Agricultural Sciences
using an insulated container with an ice pack. We extracted the nucleic
acids from the oral swab fluids and used reverse transcription PCR to
detect FMDV, VSV, BVDV, BTV, and BoHV-1 (Marlise et al., 2005) (Appendix
Table) according to the reference
protocols and SVV, including
detection and genome amplification (Table 1). We used eight cell types
from four different origins to isolate and purify the virus. We
conducted a genetic evolutionary tree based on the SVV polyprotein with
1000 bootstrap replicates using MEGA6.06 software (Neighbor-Joining).
Our results indicated that there was full-length nucleotide and amino
acid sequence similarity in the alignment between SVA/GD/China/2018 and
other 35 SVV strains published from China and other countries by
MegAlign (DNAStar Lasergene.v7.1) software using Clustal W.