Phylogenetic analysis
Phylogenetic analysis based on the nucleotide and deduced amino acid
sequences of DAstV-SDZZ isolate, was performed using ClustalW in the
Megalin module within the Lasergene 7.0 software (DNASTAR Inc., Madison
WI, USA) to determine the genetic relationships among DAstV strains.
Phylogenetic trees of the three ORFs were done by the neighbor-joining
method implemented in MEGA version 7.0 program, with bootstrap values
calculated from 1000 replicates.