Phylogenetic analysis
Phylogenetic analysis based on the nucleotide and deduced amino acid sequences of DAstV-SDZZ isolate, was performed using ClustalW in the Megalin module within the Lasergene 7.0 software (DNASTAR Inc., Madison WI, USA) to determine the genetic relationships among DAstV strains. Phylogenetic trees of the three ORFs were done by the neighbor-joining method implemented in MEGA version 7.0 program, with bootstrap values calculated from 1000 replicates.