NGS analysis
A total of 1,735,463 sequencing reads were generated by the HiSeq
sequencer from extracted viral total RNA, and yielding 376 Mb of fastq
format sequenced data. After processed by quality control (QC) filters
of the Hiseq platform, non-research target readings that were similar to
mRNA or rRNA sequences were removed. The residual 231,327 clean reads
were assembled by de novo assembly module of CLC Genomic Workbench
software (version 10.0). Finally, one long contig that covered the full
length of an astrovirus was generated by re-mapping the clean reads back
to the astrovirus reference sequences. Whereas no other pathogen was
discovered by analysing all assembled contigs.