Virus isolation
The liver sample (ZaoZhuang County) from Shandong province was selected
for virus isolation. After five passages, an astrovirus designated as
DAstV/SDZZ/Duck (DAStV-SDZZ) strain, was successfully isolated. Most
inoculated cells showed CPE after inoculation within 72–96 hr and
tested positive for DAStV-1 by RT-PCR using the above primers.