Bacterial strains and plasmids
Bacterial strains and plasmids used in this study are listed in
Supplementary Table S1. E . coli strains were cultured on
Luria–Bertani (LB) medium at 37°C. The P. ananatis PA13 was
cultivated at 28°C on LB medium. Antibiotics were used at the following
concentrations: ampicillin, 100 µg mL–1; kanamycin,
50 µg mL–1; rifampicin, 50 µg
mL–1; tetracycline, 10 µg mL–1;
gentamycin, 25 µg mL–1.
5-Bromo-4-chloro-3-indoyl-b-D-galactopyranoside (X-gal) was used at 40
µg mL–1 when necessary.