METHODS
The goal of this study was to evaluate thiol/disulphide balance as a
novel marker of OS in TB patients and to investigate changes occurring
in the oxidant antioxidant system, TDH parameters (serum native thiol,
total thiol and disulphide levels and disulphide/native thiol,
disulphide/total thiol, native thiol/total thiol ratios) measurements in
TB.
This study was performed with respect to the recommendations put forward
via the Declaration of Helsinki. The study protocol was approved by the
Ethical Committee and each participant gave written, informed consent.
50 patients with active lung tuberculosis referred to the Chest Diseases
department of Harran University Medical Faculty Hospital (29 male, 21
female) and 50 healthy controls (29 male, 21 female) were included in
the study.
Venous blood samples from the patients and healthy controls in the study
were collected. Plasma blood samples were centrifuged at 1500 rpm for 10
min and serum was obtained. The separated serum was immediately placed
in Eppendorf tubes and these samples were stored at −80°C until used.
Thiol/disulphide homeostasis evaluation had performed by a
fully‑automatic method, developed by Erel and Neselioglu [12].
Disulphide bonds are first reduced with sodium borohydride to create
functional thiol groups. Unused reducing agent, sodium borohydride, was
removed with formaldehyde to prevent reduction of
5,5’‑dithiobis‑(2‑nitrobenzoic acid) (DTNB). All thiol groups, including
reduced and native thiol groups, were later fixed by reactions with
DTNB. Half of the difference between total thiol and native thiol
determined the dynamic disulphide amount. After determining native and
total thiols, disulphide levels, disulphide/total thiol,
disulphide/native thiol, and native thiol/total thiol ratios were
calculated.