Research Article
Combinatorial analysis of host-leader-Fab can greatly improve integrated
process design
Mathias Fink1
Clemens Schimek1
Monika Cserjan-Puschmann1
Daniela Reinisch2
Cécile Brocard2
Rainer Hahn1
Gerald Striedner1
1Christian Doppler Laboratory for production of
next-level biopharmaceuticals in E. coli , Department of
Biotechnology, University of Natural Resources and Life Sciences, A-1190
Vienna, Austria
2Boehringer Ingelheim RCV GmbH & Co KG, Dr.
Boehringer-Gasse 5-11, A-1120 Vienna, Austria
Correspondence: Mrs. Monika Cserjan-Puschmann, Department of
Biotechnology, University of Natural Resources and Life Sciences, Vienna
Muthgasse 18, A-1190 Vienna, Austria.
E-mail : monika.cserjan@boku.ac.at
Keywords: E. coli ; Fab; integrated process development;
Up- & Downstream processing; Downstream manufacturability
Abbreviations: CDM, cell dry mass; Fab,antigen binding fragment; HCD, high cell density; DSP ,
downstream processing; µ-bioreactor, micro-bioreactor;QbD, quality by design; scFv, single-chain fragment
variable; USP, upstream processing