Patients samples
Altogether seven formaldehyde-fixed paraffin-embedded tissue (FFPE) samples were tested from the patient diagnosed with Rb/osteosarcoma in 2010 and followed until his death in 2019 at the Department of Pediatrics, University of Debrecen (Table 1. ). Peripheral blood samples from both parents was collected for analysing their carrier status. Sampling was agreed and supported by a written consent from both sides. All protocols have been approved by the author’s respective Institutional Review Board for human subjects (IRB reference number: 60355/2016/EKU) and conducted in accorcance with the Helsinki Declaration.