Protein in silico methods
Protein information for RB protein (P06400, RB_HUMAN) and for
hepatocyte growth factor receptor MET (P08581, MET_HUMAN) were obtained
from UniProt database and from RCSB Protein Data Bank. GOR method
version IV was used for secondary structure prediction [8]. Disorder
prediction was performed by IUPred2A web server [9]. Eukaryotic
linear motifs (ELM) were identified in the proteins by using ELM
database [10]. Stability changes upon point mutations were predicted
by I-Mutant 2.0 web server based on protein sequence, using default
parameters [11]. Protein-protein interaction data were obtained from
BioGRID [12] and STRING databases [13].