DNA isolation
DNA isolation from peripherial blood was performed using QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). Genomic DNA was extracted from FFPE tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany). The isolations were carried out according to the manufacturer’s standard protocol and the DNA was eluted in 50 µL elution buffer. The DNA concentration was measured in the Qubit dsDNA HS Assay Kit using a Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).