2.3. DNA extraction
DNA samples of all the accessions
were isolated from the young leaves by using a plant genomic DNA kit.
The DNA samples were quantified using a Nano Drop 2000 (Wilmington, USA)
spectrophotometer and checked on 1.0% (w/v) agarose gels stained with
ethidium bromide. On the basis of concentration estimations, all samples
were diluted to 20 ng/μL and stored at −20 °C.