2.3 Genetic analysis
DNA extraction and PGx analyses were performed by Labor Risch molecular
genetics laboratory, Bern-Liebefeld, Switzerland. DNA was extracted
using the QIAsymphony DSP DNA Mini Kit according to manufacturer’s
instructions. The isolated DNA was subsequently amplified by means of
the iPLEX assay which consists of multiplex-PCR, SAP reaction and iPLEX
primer extension. The modified products were then separated using the
MassARRAY MALDI-TOF System by Agena Bioscience. The analysis included
SNPs of the following genes: ABCB1, COMT, CYP1A2, CYP2B6, CYP3A4,
CYP3A5, CYP2C9, CYP2C19, CYP2D6, CYP4F2, DPYD, OPRM1, POR, SLCO1B1, TPMT
and VKORC1. A list of the tested SNPs for each gene is provided insupplementary Table S1 .