3.1. Effect of α-Klotho and LPS treatments on cell viability and cytotoxicity of primary glia culture
The different α-Klotho concentrations (0.1nM - 4 nM) did not alter cell viability or show toxicity to glial cells in both MTT and LDH assays (Fig. 1A and 1B). Regarding the LPS challenge, concentrations between 0.01 to 100 ug/mL caused no effect in MTT assay, but in the LDH assay, only concentrations of 10 and 100 ug/mL showed toxicity to glial cells when compared to the control group (Fig. 1C and 1D). Based on these data, the concentration of 1 µg/mL of LPS was chosen for the subsequent experiments.