Statistical analysis
Results are expressed as mean ± S.E.M. of the indicated number of
experiments. Statistical comparisons for α-Klotho-induced changes in
cytokines, Western blotting, and cell viability were performed by
one-way analysis of variance (ANOVA), followed by the Tukey post-test.
All analyses were performed using a Prism 9 software package (GraphPad
Software, San Diego, CA, U.S.A.). P -values < 0.05. were
considered to reflect a statistically significant difference.