Statistical analysis
Results are expressed as mean ± S.E.M. of the indicated number of experiments. Statistical comparisons for α-Klotho-induced changes in cytokines, Western blotting, and cell viability were performed by one-way analysis of variance (ANOVA), followed by the Tukey post-test. All analyses were performed using a Prism 9 software package (GraphPad Software, San Diego, CA, U.S.A.). P -values < 0.05. were considered to reflect a statistically significant difference.