The Real-Time PCR procedure was preceded by standard curve preparation. The Real-Time PCR programs consisted of the Uracil-DNA glycosylase activation step at 50°C for 2 min, initial denaturation at 95°C for 2 min followed by 45 cycles of denaturation at 95°C for 15 sec and annealing at 60°C for 1 min. The last step was the melting curve performed to ensure reaction specificity. Specific expression levels were calculated using the 1/∆Ct algorithm, where ΔCt is the Ct value of the target splicing variant minus the mean of Ct value of reference genes.