Reverse transcription
RNA extraction was followed by gDNA elimination and reverse
transcription performed using QuantiTect Reverse Transcription Kit
(Qiagen, Hilden, Germany). 1 μg of RNA was transcribed. All reactions
were prepared on ice. Incubations during the reverse transcription
procedure were conducted in Labcycler 48 (SensoQuest GmbH, Göttingen,
Germany). Obtained cDNA was stored at -80°C temperature.