Reverse transcription
RNA extraction was followed by gDNA elimination and reverse transcription performed using QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). 1 μg of RNA was transcribed. All reactions were prepared on ice. Incubations during the reverse transcription procedure were conducted in Labcycler 48 (SensoQuest GmbH, Göttingen, Germany). Obtained cDNA was stored at -80°C temperature.