Placental samples
Placental tissue samples were collected directly after the third stage
of labour. Samples were taken from four, unaffected parts of the
placenta, one from each quadrant. The tissue was placed in a tube and
stored at -80°C until the analysis. CB expression was compared to
reference proteins glyceraldehyde 3-phosphate dehydrogenase and β-actin.
RNA isolation, then reverse transcription, and finally, Real-Time PCR
was conducted to calculate the expression of the mRNA of the examined
genes.