Real-Time PCR procedure was performed in a thermal cycler Roche LightCycler® 480 Real-Time PCR System (Roche Diagnostics Ltd, Basel, Switzerland) with the use of Power-Up SYBR Green Master Mix (Applied Biosystems, California, USA). The target genes in our study were CNR1, CNR1a, and CNR2 encoding CBR1, CBR1a, and CBR2, respectively. ACTB (β-actin) and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) served as reference genes. In a single reaction, 1 µl of cDNA was used. Gene-specific primers projected in Primer-BLAST were used at a 10-µM concentration (Primer sequences are presented in Table 1).