2.7 Luciferase Reporter Assay
The pGL4.17-COL1A1 luciferase reporter plasmid is gift from Hongwei He (Peking Union Medical College, Beijing)(Zhao, Wang, Wang, Shao & He, 2015). LX-2 cells were transfected with pGL4.17-COL1A1 and pGMGLI-Lu luciferase reporter plasmid (Yesen,11524ES03) then treated with PB (0.25, 0.5 and 1 μM) for 24 h. Cells were lysed and subjected to luciferase reporter assay by using DualLuciferase® Reporter Assay System (Vazyme, #DL101-01).