2. Materials and Methods
2.1 Materials: Zinc nitrate hexahydrate, Tween-80, ammonium carbonate, sodium hydroxide, and ethanol of analytical grade were purchased from Loba Chemie Private Limited. Dulbecco’s Modified Eagle medium (DMEM), fetal bovine serum, MitoSOX Red, Propidium Iodide, Calcein, Poly-L-lysine solution, and PBS buffer were procured from Sigma- Aldrich.