R. pedestris.
Figure 2. Identification of chromosomes in Riptortus pedestris. (A) DNA interaction heatmap generated in Hi-C interaction analysis. The shading gradient represents the chromosome interactions. (B) Chromosomic staining of female and male salivary gland cells inR. pedestris . The blue signal indicates the condensed chromosomes.
Figure 3. Landscape of the assembled Riptortus pedestrisgenome. From the outside in, the first circle shows the 6 chromosomes. The second circle showed the GC contents. The third and fourth circle showed the female and male MRPM value, respectively. The fifth circle showed the ratio of MRPM between female and male. The sixth circle showed the transcriptional reads from 37 different development stages. All data was calculated on a 1-Mb scale.
Figure 4. Analysis of Serratia marcescens Rip1. (A) Landscape of the assembled Serratia marcescens Rip1 genome. From the outside in, the first circle showed the nucleotide sequence positions (in Mb). The second circle showed the virulence factors and antibiotic-resistance genes in the genome. The third and fourth circle showed the predicted genes that transcribed in clockwise and anticlockwise, respectively. The fifth circle showed the GC contents on a 10-Kb scale. rfaF, ADP-heptose-LPS heptosyltransferase II; gpt, glycerol-3-phosphate transporter; PmrB, two-component system sensor histidine kinase BasS; alaS, aminocoumarin resistant gene; kdpE, two-component system response regulator KdpE ; fbpC, Iron (III) ABC transporter; galE, UDP-glucose 4-epimerase; msbA, Lipid transporter ATP-binding/permease; mfd, transcription-repair coupling factor; shuU, ABC transport system; SRT-2, class C beta-lactamase; cysB, aminocoumarin resistant transcriptional regulator CysB; flg, flagella synthesis genes; katG , catalase/peroxidase HPI ; omp36 , porin OmpC; fepG, Iron-enterobactin ABC transporter permease; lpxD, UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase; AAC(6’)-Ic, aminoglycoside N-acetyltransferase AAC(6’)-Ic. (B) Phylogenetic relationship between Serratiaspecies.
Figure 5.Phylogenetic analysis ofRiptortus pedestris with other insect species. The phylogenetic position of R. pedestris was determined based on 963 single copy genes with 356,827 reliable sites. D. melanogaster was used to root the tree. 1:1:1 indicates single copy genes, and N:N:N indicates multicopy genes across 14 insect species. The Sternorrhyncha, Auchenorrhyncha, and Heteroptera indicate suborder‐specific genes, respectively. The estimated species divergence time is illustrated at the bottom of the phylogenetic tree. Gene family expansion (red) and contraction (green) are illustrated at the branches and nodes of the tree.
Figure 6. Genome synteny between Triatoma rubrofasciataand Riptortus pedestris. Synteny analysis demonstrated fusion or fission events between the two bug species.