Figure 3. Production of Z 11-14:OH in small scale
cultivations and in 1 L fed-batch fermentation. A. Metabolic
engineering of Y. lipolytica for improved production ofZ 11-14:OH. High myristate producing strain ST7982, containing
mutation in α-subunit of the fatty acyl synthase complex
(Fas2pI1220F) was used as background strain. Results
are obtained from three biological replicates. B. Fed-batch
fermentation results of ST9253. Data obtained for optical density (OD),
cell dry weight (DW), glycerol, and Z 11-14:OH concentration are
represented as averages from three bioreactors and standard deviations
are calculated which are shown as error bars.