Supporting Information Legends:
Figure S1: Sample-to-sample distance heatmap . Sample-to-sample distance heatmaps for the comparison between different sampling techniques and different tissue harvesting time for the different tissues. The rows and columns are arranged based on hierarchical clustering, so that samples with similar expression profiles are positioned near to each other. The color scale represents the distance between samples. A value of distance 0 indicates that two samples have identical gene expression. The smaller the distance is, the higher is the correlation between two samples. Treatment groups compared (called “condition ”) are indicated in different colors next to each heatmap. Group 1 = fish captured by dip netting, Grou[ 2 = fish captured by electrofishing processed immediately, Group 3 = fish captured by electrofishing processed 5 minutes after euthanasia.A. QuantSeq dip netting versus electrofishing only for blood samples, B. QuantSeq dip netting versus electrofishing only for gill samples, C. QuantSeq dip netting versus electrofishing only for muscle samples, D. QuantSeq electrofishing with immediate sampling versus electrofishing with delayed sampling only for blood samples, E. QuantSeq electrofishing with immediate sampling versus electrofishing with delayed sampling only for gill samples, F. QuantSeq electrofishing with immediate sampling versus electrofishing with delayed sampling only for muscle samples.
Figure S2: PCA plots showing PC1 and PC2 for samples that are differentially expressed among sampling techniques and tissue harvesting time for the different tissues. A. QuantSeq dip netting versus electrofishing only for blood samples, B. QuantSeq dip netting versus electrofishing only for gill samples, C.QuantSeq dip netting versus electrofishing only for muscle samples,D. QuantSeq electrofishing with immediate sampling versus electrofishing with delayed sampling only for blood samples, E.QuantSeq electrofishing with immediate sampling versus electrofishing with delayed sampling only for gill samples, F. QuantSeq electrofishing with immediate sampling versus electrofishing with delayed sampling only for muscle samples.