3.3.1 Dip Netting versus electrofishing sampling method
QuantSeq data identified only 3 out of 39,212 genes (0.008%) that were significantly (adjusted p- value < 0.05) differentially expressed between dip netting and electrofishing across all tissue types. When gene expression between dip netting and electrofishing was analyzed separately for different tissue types, 0 out of 27,118 genes were differentially expressed in blood, 16 out of 20,465 genes (0.08%) in gills, and 155 out of 9,201 genes (1.7%) in muscle. NEB data (available for blood samples only) confirmed QuantSeq data, with no genes showing differential expression between the two sampling methods (0 out of 33,236 genes) (Table S2 on Dryad).