Supporting Information Legends:
Figure S1: Sample-to-sample distance heatmap .
Sample-to-sample distance heatmaps for the comparison between different
sampling techniques and different tissue harvesting time for the
different tissues. The rows and columns are arranged based on
hierarchical clustering, so that samples with similar expression
profiles are positioned near to each other. The color scale represents
the distance between samples. A value of distance 0 indicates that two
samples have identical gene expression. The smaller the distance is, the
higher is the correlation between two samples. Treatment groups compared
(called “condition ”) are indicated in different colors next to
each heatmap. Group 1 = fish captured by dip netting, Grou[ 2 = fish
captured by electrofishing processed immediately, Group 3 = fish
captured by electrofishing processed 5 minutes after euthanasia.A. QuantSeq dip netting versus electrofishing only for blood
samples, B. QuantSeq dip netting versus electrofishing only for
gill samples, C. QuantSeq dip netting versus electrofishing
only for muscle samples, D. QuantSeq electrofishing with
immediate sampling versus electrofishing with delayed sampling only for
blood samples, E. QuantSeq electrofishing with immediate
sampling versus electrofishing with delayed sampling only for gill
samples, F. QuantSeq electrofishing with immediate sampling
versus electrofishing with delayed sampling only for muscle samples.
Figure S2: PCA plots showing PC1 and PC2 for samples
that are differentially expressed among sampling techniques and tissue
harvesting time for the different tissues. A. QuantSeq dip netting
versus electrofishing only for blood samples, B. QuantSeq dip
netting versus electrofishing only for gill samples, C.QuantSeq dip netting versus electrofishing only for muscle samples,D. QuantSeq electrofishing with immediate sampling versus
electrofishing with delayed sampling only for blood samples, E.QuantSeq electrofishing with immediate sampling versus electrofishing
with delayed sampling only for gill samples, F. QuantSeq
electrofishing with immediate sampling versus electrofishing with
delayed sampling only for muscle samples.