Introduction
The role of zoological collections in biosurveillance has been proven in the past (McNamara, 2007). Many different animal species usually live in a small area and in proximity to keepers, visitors, and other wildlife. In some situations, infectious pressure might overcome the immune defense of zoo animals, and pathogens might spread across various animal species. In this context, infectious diseases monitoring is an extremely important part of preventive veterinary medicine in zoological collections. A well-designed vaccination program and regular coprological examinations, including flotation and sedimentation techniques, are obligatory in order to provide good health care for animals in the zoo. A detailed screening plan for selected pathogens based on the current epidemiological situation is of imminent importance for the safety of animals and employees, as well as visitors. The possibility of discovering infectious threats such as toxoplasmosis, neosporosis, chlamydiosis, and encephalitozoonosis makes zoos suitable epidemiological stations. The aim of this study was to monitor selected pathogens at the Ljubljana Zoo in Slovenia and examine its role as a sentinel in biosurveillance.