3.2 Compactional screening the mutant candidates with better
catalysis efficiency and thermostability
The structures of α-L-rhamnosidase Rha1 (GenBank code: AGN92963.1) and
its mutants were obtained by homology modeling using Modeller 9.15.
Energy minimization was carried out using Discovery Studio2019. The
three-dimensional structure model of p NPR was contructed by Chem
Bio Office 2017. The simulated protein structure was docked againstp NPR using AutoDock4.2. In general, the docking parameters for
AutoDock4.2 were kept as default values. [(145.704, 166.348, 34.581),
(60 Å×60 Å×60 Å)] grid map was used in docking calculations. Finally,
the best poses were analyzed and visualized using Discovery studio 2019.