2.2. Animals
All animal care and experimental procedures were in accordance with the current edition of the NIH Guide for the Care and Use of Laboratory Animals and were approved by the local Animal Ethical Committee (CEUA-UFMG; protocol 213/2016). Adult male Wistar rats (8-12 weeks old; weighting 200-250 g), neonatal male Wistar rats (3-5 days old) and adult male C57Bl/6 mice (8-12 weeks old; weighting 20-24 g) were obtained from the CEBIO-UFMG. B1 and B2 receptor knockout (B1RKO and B2RKO, respectively) male mice were obtained from the animal facility of the Department of Biochemistry and Immunology at UFMG. All animals were housed in standard cages at the animal facility of the Department of Physiology and Biophysics (UFMG), with controlled temperature (22 ± 2o C) and under a 12/12 h light-dark cycle. Standard chow and filtered tap water were available ad libitum . Animal studies were done in accordance with the ARRIVE guidelines (Kilkennyet al. , 2010), and with the recommendations made by the editorial board of the British Journal of Pharmacology (Lilley et al., 2020). Animals were divided randomly in the experimental groups and the investigator involved in the data analyses was blinded to the experimental conditions.