2.2. Animals
All animal care and experimental procedures were in accordance with the
current edition of the NIH Guide for the Care and Use of
Laboratory Animals and were approved by the local Animal Ethical
Committee (CEUA-UFMG; protocol 213/2016). Adult male Wistar rats (8-12
weeks old; weighting 200-250 g), neonatal male Wistar rats (3-5 days
old) and adult male C57Bl/6 mice (8-12 weeks old; weighting 20-24 g)
were obtained from the CEBIO-UFMG. B1 and
B2 receptor knockout (B1RKO and B2RKO, respectively)
male mice were obtained from the animal facility of the Department of
Biochemistry and Immunology at UFMG. All animals were housed in standard
cages at the animal facility of the Department of Physiology and
Biophysics (UFMG), with controlled temperature (22 ± 2o C) and under a 12/12 h light-dark cycle. Standard
chow and filtered tap water were available ad libitum . Animal
studies were done in accordance with the ARRIVE guidelines (Kilkennyet al. , 2010), and with the recommendations made by the editorial
board of the British Journal of Pharmacology (Lilley et al.,
2020). Animals were divided randomly in the experimental groups and the
investigator involved in the data analyses was blinded to the
experimental conditions.