2.11 Data and statistical analyses
Experimental design and statistics were as recommended by theBritish Journal of Pharmacology (Curtis et al. , 2018). All
experiments were done using 5 or 8 independent replicates (n = 5 or n =
8). Results are expressed as means ± SEM. All statistical analyses were
performed with GraphPad Prism v.7.0 and P < 0.05 was
considered to show statistical significance between group means.
Confocal microscopy (n = 5), plasma extravasation (n= 8) and nociception
experiments (n = 8) were analysed by one-way ANOVA. MS-based plasma
peptide quantitation (n= 5), vascular reactivity experiments and mean
arterial pressure (MAP, n = 5) and heart rate (HR, n = 5) measurements
were analysed by two-way ANOVA. Relative mRNA quantitation (qPCR, n= 5)
data were analysed by unpaired t test (parametric test due to normal
distribution).