Study limits
Our results are limited by several factors. This preliminary study was
conducted with few individuals (1-2) for each population, and as such
should be considered a baseline for future studies. The available sample
sizes for every population is ten individuals. We hope to conduct future
studies with all the individual samples.
Social instability was the main reason no samples of L.
columnaris were collected in Nigeria. Future fieldwork in Nigeria to
find and collect more populations will expand the sampling effort and
allow a more robust phylogeographic study. Populations from Mt. Cameroon
were under-collected because it was challenging to get the permits to
work in the National Park. Finally, this study presents a partial
history with the analysis of a non-recombinant marker. A more robust
analysis should include nuclear genomic sequences.