3.5 Evaluating consistency between the LAMP-CRISPR assay and the commercial qPCR kit in porcine from clinical samples
To evaluate the performance of the LAMP-CRISPR assay, 41 clinical samples including nasal swab, spleen, liver, lung, submandibular lymph node and kidney were assessed by both the LAMP-CRISPR and the commercial qPCR assay. As shown in Table 2, 5 out of 6 nasal swabs, 5 out of 7 spleens, 2 out of 7 livers, 6 out of 7 lungs, 5 out of 7 submandibular lymph nodes and 5 out of 7 kidney samples tested positive in both LAMP-CRISPR and qPCR assays, respectively (Figure 5). While only one more sample was positive in the liver when tested with the qPCR assay. The Ct value of that positive sample for qPCR is 36.12, which indicated the number of virus copies from that sample is quite low. This result showed 96.6% consistency with these two assays, which supports LAMP-CRISPR can be regarded as a novel diagnostic assay for the detection of ASFV.