3.5 Evaluating consistency between the LAMP-CRISPR assay and the
commercial qPCR kit in porcine from clinical samples
To evaluate the performance of the LAMP-CRISPR assay, 41 clinical
samples including nasal swab, spleen, liver, lung, submandibular lymph
node and kidney were assessed by both the LAMP-CRISPR and the commercial
qPCR assay. As shown in Table 2, 5 out of 6 nasal swabs, 5 out of 7
spleens, 2 out of 7 livers, 6 out of 7 lungs, 5 out of 7 submandibular
lymph nodes and 5 out of 7 kidney samples tested positive in both
LAMP-CRISPR and qPCR assays, respectively (Figure 5). While only one
more sample was positive in the liver when tested with the qPCR assay.
The Ct value of that positive sample for qPCR is 36.12, which
indicated the number of virus copies from that sample is quite low. This
result showed 96.6% consistency with these two assays, which supports
LAMP-CRISPR can be regarded as a novel diagnostic assay for the
detection of ASFV.